CoMakingSpace Wiki - Recent Changes [en]

Project:Digital Holographic Miocroscope

Lukas — Thu, 20 Aug 2026 20:29:27 GMT

Lukas moved page Project:Digital Holographic Miocroscope to Project:Digital Holographic Microscope without leaving a redirect typo

New page

{{DISPLAYTITLE:Project:Digital Holographic Microscope}}
{{ProjectInfoBox
|project title=Digital Holographic Microscope
|image=Holmos setup comakingspace.jpeg
|status=being built
|date=
|initiator=[[User:P|Patrick]], [[User:Lennart|Lennart]]
|team=
|materials=
|tools=
|software=
|cost=
}}

=== Introduction ===

==== Holmos ====
This project is based on project Holmos (https://github.com/holmos-ipm) by Fraunhofer IPM.

==== Digital Holographic Microscopy ====
Wikipedia https://en.wikipedia.org/wiki/Digital_holographic_microscopy

This file explains the constraints in optical adjustment https://github.com/holmos-ipm/holmos-hardware/blob/master/instructions/optics_design.md

=== Assembly ===
Mounts for the optical components can be printed from files provided in the project repo. These can then be axially moved on metal beams. A Raspberry Pi camera is used for imaging. It is connected to a Raspberry Pi that performs the phase image reconstruction.
[[File:Holmos objective mount stl.png|thumb|139x139px|Several components can be 3D-printed either from ready-to-use STL files or 3D files generated with Solidpython ]]

=== Usage ===
Key steps are

# Position the components according to https://github.com/holmos-ipm/holmos-hardware/blob/master/reference_assembly.py
# Adjust slide holder or objective to get a focused image from the objective beam only
# Direct the reference beam onto the camera sensor

Further practical instructions are given in https://github.com/holmos-ipm/holmos-hardware/blob/master/instructions/adjustment.md

In the Holmos Fourier amplitude view, the first diffraction order is selected as ROI. Then, the captured ("wrapped") phase image is saved on the hard drive. In a separate application, this image is then "unwrapped", e.g. black to white jumps due to the restricted value range of -π to π are removed.
[[File:Holmos emulator.jpg|thumb|363x363px|Fourier amplitude view in the Holmos UI. The blue rectangle is moved to encompass the first diffraction order and surrounding frequences stemming from the interference of the reference beam and light passing the sample.|none]]

=== Results ===
<gallery widths="140" heights="140" perrow="2" caption="Quantitative phase image reconstructed from a cheek cell hologram.">
File:Holmos cheek interference.png|Interference image captured by the camera
File:Holmos cheek fft.png|Fourier amplitude in which the information of interest is encoded in the frequencies around the first diffraction order
File:Holmos cheek wrapped.png|Wrapped phase image reconstructed from the ROI in the Fast Fourier Transform
File:Holmos cheek unwrapped.png|Unwrapped phase image
</gallery>

Project:Digital Holographic Miocroscope

Lennart — Thu, 20 Aug 2026 18:05:37 GMT

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			{{DISPLAYTITLE:Project:Digital Holographic Microscope}}
	{{ProjectInfoBox		{{ProjectInfoBox
	\|project title=Digital Holographic Microscope		\|project title=Digital Holographic Microscope
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	File:Holmos cheek unwrapped.png\|Unwrapped phase image		File:Holmos cheek unwrapped.png\|Unwrapped phase image
	</gallery>		</gallery>

	~~=== Challenges ===~~

KiCad Seminar 2026

Lmnl99 — Wed, 19 Aug 2026 15:06:58 GMT

← Older revision		Revision as of 17:06, 19 August 2026
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	== Mitbringen ==		== Mitbringen ==
	Bitte bring einen '''Laptop''' mit, auf dem '''[https://www.kicad.org/ KiCad 9] bereits installiert''' ist, sowie eine Maus (am besten mit Mausrad).		Bitte bring einen '''Laptop''' mit, auf dem '''[https://www.kicad.org/ KiCad 10] bereits installiert''' ist, sowie eine Maus (am besten mit Mausrad).

	'''Der KiCad-Download ist groß – bitte unbedingt vorher zuhause installieren, das schaffen wir vor Ort nicht.''' Falls du bei der Einrichtung (Bibliotheken, Konfiguration) Hilfe brauchst, komm einfach '''45 Minuten früher''', dann richten wir das gemeinsam ein. Wer ganz ohne lauffähiges KiCad kommt, kann leider nicht mitmachen, weil ich während des Seminars keine Komplett-Installationen betreuen kann.		'''Der KiCad-Download ist groß – bitte unbedingt vorher zuhause installieren, das schaffen wir vor Ort nicht.''' Falls du bei der Einrichtung (Bibliotheken, Konfiguration) Hilfe brauchst, komm einfach '''45 Minuten früher''', dann richten wir das gemeinsam ein. Wer ganz ohne lauffähiges KiCad kommt, kann leider nicht mitmachen, weil ich während des Seminars keine Komplett-Installationen betreuen kann.

Handheld Router Introduction

Lukas — Wed, 19 Aug 2026 11:58:29 GMT

updated requirement sentence

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	If you need this introduction, please reach out to the following tutors: [[Tutor::User:Lukas\|Lukas]], [[Tutor::User:NitramLegov\|Martin]], [[Tutor::User:Luzian\|Luzian]], [[Tutor::User:Keno\|Keno]], [[Tutor::User:Johannes\|Johannes]], [[Tutor::User:Pakue\|Patrick]], [[Tutor::User:Utis\|Utis]], [[Tutor::User:Mobell\|Moritz]], [[Tutor::User:Björn\|Björn]], [[Tutor::User:Jaggi2\|Jakob S.]]		If you need this introduction, please reach out to the following tutors: [[Tutor::User:Lukas\|Lukas]], [[Tutor::User:NitramLegov\|Martin]], [[Tutor::User:Luzian\|Luzian]], [[Tutor::User:Keno\|Keno]], [[Tutor::User:Johannes\|Johannes]], [[Tutor::User:Pakue\|Patrick]], [[Tutor::User:Utis\|Utis]], [[Tutor::User:Mobell\|Moritz]], [[Tutor::User:Björn\|Björn]], [[Tutor::User:Jaggi2\|Jakob S.]]

	Please be aware that the ~~[[CNC Router Introduction\|CNC router introduction]] and~~ [[Router Table Introduction\|router table introduction]] (as of 2020-4-15) ~~are '''separate'''~~ but ~~require~~ this one as a background.		Please be aware that the [[Router Table Introduction\|router table introduction]] is '''separate''' (as of 2020-4-15) but requires this one as a background.

	First off, make sure you have read the tool's manual, if available (it should be linked from its InfoBox).		First off, make sure you have read the tool's manual, if available (it should be linked from its InfoBox).

CNC Mill Introduction

Lukas — Wed, 19 Aug 2026 11:57:56 GMT

replaced CNC router requirement with recommendation

← Older revision		Revision as of 13:57, 19 August 2026
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	If you need this introduction, please reach out to the following tutors: [[Tutor::User:Sebastian\|Sebastian]], [[Tutor::User:Luzian\|Luzian]]		If you need this introduction, please reach out to the following tutors: [[Tutor::User:Sebastian\|Sebastian]], [[Tutor::User:Luzian\|Luzian]]

	Before you get this introduction, ~~please make sure~~ you ~~already have~~ the [[Handheld Router Introduction]], this will help you understand the machine much better ~~and is required before using it~~. For CNC beginners, it is recommended that you start with the [[CNC Router Introduction\|CNC '''router''' introduction]] and gain your first experience on the [[Workbee CNC]].		Before you get this introduction, we recommend you get the [[Handheld Router Introduction]] and gather some experience, this will help you understand the machine much better. For CNC beginners, it is recommended that you start with the [[CNC Router Introduction\|CNC '''router''' introduction]] and gain your first experience on the [[Workbee CNC]].

	== Safety ==		== Safety ==
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	* Even with the rail, chips can fly towards you. You MUST stay clear and you MUST wear eye protection.		* Even with the rail, chips can fly towards you. You MUST stay clear and you MUST wear eye protection.
	\|}		\|}

			=== [[Feedrate]] ===
			The proper combination of rotation speed and tool advancement is determined by your bit and material.
			* be cautions not to remove too much material at a time - several passes are usually necessary if you want to go deep
			* advancing ''too slow'' can produce excessive heat, advancing too fast can cause the workpiece to chip and even damage the bit

	== Suggested Reading ==		== Suggested Reading ==

CNC Router Introduction

Lukas — Wed, 19 Aug 2026 11:55:44 GMT

Safety: formatting

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	\|\| when you see smoke, immediately stop and re-think your tool settings - it might be spinning too fast or you could be feeding too slow! Are you sure your bit is sharp?		\|\| when you see smoke, immediately stop and re-think your tool settings - it might be spinning too fast or you could be feeding too slow! Are you sure your bit is sharp?
	\|-		\|-


	\| [[File:Danger_-_hand injury.svg\|left\|50px]] the machine has no live sensors and will execute its code regardless of what gets into its way		\| [[File:Danger_-_hand injury.svg\|left\|50px]] the machine has no live sensors and will execute its code regardless of what gets into its way
	* heat generated during improper use can even [https://www.youtube.com/watch?v=4tpxgZUYGSI set the machine on fire] [[File:danger - fire.svg\|50px]]		* heat generated during improper use can even [https://www.youtube.com/watch?v=4tpxgZUYGSI set the machine on fire] [[File:danger - fire.svg\|50px]]
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	\|}		\|}

	==== Feed Direction ====		=== Feed Direction ===
	The feed direction at this CNC router cannot be influenced. Lots of CNC routers allow to set the feed direction using G-codes [https://docs.duet3d.com/User_manual/Reference/Gcodes M3 and M4]. On the Workbee a handheld router is mounted which cannot change its spinning direction, it always use M3 (clockwise). The CNC router thus does push and climb cuts at the same work piece depending on your geometry.		The feed direction at this CNC router cannot be influenced. Lots of CNC routers allow to set the feed direction using G-codes [https://docs.duet3d.com/User_manual/Reference/Gcodes M3 and M4]. On the Workbee a handheld router is mounted which cannot change its spinning direction, it always use M3 (clockwise). The CNC router thus does push and climb cuts at the same work piece depending on your geometry.

	==== [[Feedrate]] ====		=== [[Feedrate]] ===
	The proper combination of rotation speed and tool advancement is determined by your bit and material.		The proper combination of rotation speed and tool advancement is determined by your bit and material.
	* be cautions not to remove too much material at a time - several passes are usually necessary if you want to go deep		* be cautions not to remove too much material at a time - several passes are usually necessary if you want to go deep

CNC Mill Introduction

Lukas — Wed, 19 Aug 2026 11:51:55 GMT

Safety: added general router bit/end mill safety

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	! Dangers !! Precautions		! Dangers !! Precautions
	\|-		\|-
	\| sharp, fast spinning bits ([[End Mills\|end mills]] or - rarely - certain [[Router Bits\|router bits]]) \|\|		\| [[File:Danger_-_draw-in.svg\|left\|50px]] sharp, fast spinning bits ([[End Mills\|end mills]] or - rarely - certain [[Router Bits\|router bits]]) \|\|
	* You MUST stay clear of the spindle when in operation		* You MUST stay clear of the spindle when in operation
	* You MUST unplug the spindle when changing a bit or touching the chuck for any other reason		* You MUST unplug the spindle when changing a bit or touching the chuck for any other reason
			* insert your mill as far as you can afford for maximum strength
	\|-		\|-
	\| the bit may break during operation, sending part(s) of it flying toward you \|\|		\| the bit may break during operation, sending part(s) of it flying toward you \|\|
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	* You SHOULD position the wooden or acrylic U-shaped box over the work area; the dust shoe also offers some protection		* You SHOULD position the wooden or acrylic U-shaped box over the work area; the dust shoe also offers some protection
	* You SHOULD NOT rapidly cool a hot bit by suddenly applying coolant - rather, cool continuously or do not cool at all		* You SHOULD NOT rapidly cool a hot bit by suddenly applying coolant - rather, cool continuously or do not cool at all
			\|-
			\|the bit can get extremely hot
			* can potentially set sawdust on fire
			* remains hot for a while after using
			\|\| when you see smoke, immediately stop and re-think your tool settings - it might be spinning too fast or you could be feeding too slow! Are you sure your bit is sharp?
	\|-		\|-
	\| strong forces acting on the workpiece that might make it move \|\|		\| strong forces acting on the workpiece that might make it move \|\|